Wheat (Triticum aestivum L.) is an important cereal crop of Pakistan. The present experiment was designed to screen advance bread wheat genotypes against stripe rust resistance. A set of 93 bread wheat genotypes including three check cv. Kohat-2017, Pakistan-2013 and Morocco (susceptible check) were planted in augmented design as observational nursery with zero replications across three locations of Khyber Pakhtunkhwa i.e., Kohat (E-01), Nowshera (E-02) and Peshawar (E-03). Among 93 genotypes, five lines showed 0-TR reaction with CI values of 0, 39 lines showed MR reaction with CI values between 5 to 12, 11 lines showed R type of reaction with CI value between 1 to 2, 29 lines exhibited MR-MS or M reaction with CI values between 18 to 24, 2 lines showed MS reaction with CI value of 24, one line showed MSS reaction with CI value of 36, while six lines including Morocco expressed S reaction with CI value between 60-90 at Kohat. Similarly, at Nowshera, six lines exhibited 0-TR reaction with CI values of 0, one line showed R type reaction with CI value of 4, 10 line expressed MR reaction with CI values between 2 to 8, 69 lines showed M type reaction with CI values between 3 to 24, 6 lines exhibited MS reaction with CI value between 24 to 48, while Morocco showed S type reaction with CI value of 100. Likewise, 11 lines showed 0 type reactions with CI values of 0, 11 lines exhibited R type reaction with CI values of 1, 11 lines expressed MR reaction with CI values between 4 to 16, four lines displayed MS reaction with CI values between 24 to 40, 55 lines expressed M type reactions with CI values between 3 to 24, while Morocco showed S reaction with CI value of 90 at Peshawar. SSR marker data revealed that 16 lines including check cv. Morocco showed absence of both Yr5 and Yr10 gene, 17 lines including check cv. Kohat-2017 expressed the presence of Yr5 gene only, 9 lines including Pakistan-2013 carries Yr10 gene only, while 49 line carry both Yr5 and Yr 10 genes. Combining molecular phenotypic data, among top 25 lines based on ACI values nine lines carrying both Y5 and Yr10 genes showed high resistance to stripe rust exhibiting lower ACI values, while nine lines carrying Yr5 and three lines carrying only Yr10 gene only also showed desirable lower ACI value indicating effectiveness of these genes against stripe rust. Surprisingly, four lines showed desirable lower ACI values without carrying Yr5 and Yr10 showing the presence of unique genes in their genetic makeup.
