The root lesion nematodes (Pratylenchus neglectus and P. thornei) are major pathogen of cereals in many regions worldwide. Wheat genotypes resistance to these nematodes can be determined from final nematode population densities in controlled environmental chambers or glass house. To assess the prevalence of root lesion nematodes in wheat experimental field area of National Agricultural Research Center, Islamabad, soil samples along with wheat seeding of fifty seven historical wheat genotypes were collected in 2022. The nematode species P.neglectus was found in all the wheat genotypes associated with roots as well as in root rhizosphere. For fields having root lesion nematodes in 2022, P. neglectus mean population densities were found Margalla variety with maximum nematode population (2033/100grsms) of soil, followed by wheat varieties as Markaz-19 (1446/100grams), Bakhtawar (1320/100grams), Suleman-96 (1140/100grams), Johar-16 (1053/100grams), Pastor (1040/100grams), Zinkol-2016, a zinc fortified wheat variety (1026/100grams) and WL711 (1000/100grams). Stunt nematodes (Tylenchorhynchus spp) were also detected from all the root rhizosphere in association.