The stripe (Yellow) rust of wheat is one of the wrecker diseases in Iran. Since 1993 several epidemics have occurred in Iran causing the breakdown of widely utilized sources of resistance in wheat cultivars. Sixtey seven isolates were collected from different parts of Iran during 2018-2020. Infection types were assessed on a 0-9 scale 16 and 18 days after inoculation using a scale similar to that described by McNeal et al. (1971). Infection types (ITs) 7 to 9 were regarded as virulent (susceptible) and less than 7 are avirulent. Pathotypes 238E182A+,Yr27 (from Brojerd) and 247E235A+,Yr27+ (from Saraks 2-3) were more aggressive during this study. Pathotype 7E0A-(Arak) and 6E6A+ (Bostanabad) were less aggressive. Plants with gene/s Yr5,Yr15 and Yr24 were resistant to all pathotypes. Virulence on plant with gene/s Yr2, Yr6, Yr7, Yr9, Yr25, Yr27,YrSD and YrA were more common, which are detected in greenhouse condition.