In 2016 and 2017, 25 Berberis plants in Sinop province located in northern Turkey was examined for the presence of aecial infection. The elevation of Berberis plants ranged between 334-543 m. The estimated number of Berberis plants per 100 m2 ranged between 1 and 30 and the number of infected plants in 10 m ranged between 1-10. The heights of the Berberis plants were between 1.50-2.10 m. Aecial infection was observed in 9 Berberis plants. In these plants, percentages of aecial infection varied between 2-90. No fruits were observed in one Berberis plant, however, other Berberis plants formed dark blue-black fruits. These Berberis plants were identified as Berberis crataegina based on morphological characters. From three B. crataegina plants, 17 single aecial pustules were obtained and inoculated on the North American differential set. Fifteen isolates were stem rust race TTTTF and 2 isolates were stem rust race TTKTF. Detailed studies are necessary for the B. crataegina-pathogen interaction.